Trichomonas vaginalis : Orange-red with the yellow-green nucleus Results interpretation of Acridine orange Add a drop of saline or distilled water to the smear, and cover with a cover glass.Įxamine first with the 10X objective to see the distribution of fluorescing material, and then with the 40X objective to identify Trichomonas vaginalis and to detect yeast cells, bacteria, and parasites( chromatoid bars of Entamoeba histolytica/ dispar).Rinse the smear with physiological saline, and place the slide in a draining rack.
Now, wash off the stain, and decolorize the smear with alcohol saline solution for 5–10 seconds.Cover the unfixed dried smear with the acridine orange acid stain for 5–10 seconds and smear fixes due to being fixative is contained in the stain.The procedure of Acridine orange staining Fluorescence microscope having a BG 12 exciter filter and No.Inoculating loop or sterile bamboo stick.It can also use to detect intracellular gonococci, meningococci, and other bacteria, particularly in blood cultures.Īcridine orange is a fluorochrome that causes DNA to fluoresce green and Acridine Orange Stain Introduction of Acridine orangeĪcridine orange is a fluorochrome stain and uses the rapid identification of Trichomonas vaginalis, yeast cells, and clue cells in vaginal smears. ® credit.Stain in Microbiology chapter contains nearly all the stains those applicable in microbiology that contains name list of stains, introduction, uses, in some principle, procedure, result in interpretation, keynotes, etc.
This course is approved for 1.5 contact hours of P.A.C.E. The Centers for Disease Control and Prevention, Division of Laboratory Systems is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.
Laboratory professionals should have basic knowledge and understanding of routine microscopy procedures and techniques.
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